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棉花花药愈伤组织培养与胚胎发生
ANTHER CALLUS CULTURE AND EMBRYOGENSIS OF COTTON
【摘要】 棉花花药愈伤组织的生长与增殖受基因型、糖源、培养基成分等多种因素的影响。经多次继代筛选后,棉花花药愈伤组织中可形成一种能分化出胚状体的胚性愈伤组织,并能产生不同发育时期的胚状体。胚状体转入胚荫发培养基中,可萌发形成小植株。本实验获得了鲁棉6号小植株和Siokral-3及珂字201的于叶胚。
【Abstract】 Growth and proliferation of cotton anther callus were affected by many factors,such as.genotypes,sugars or the composition of medium. Embryogenic callus took shape after timesof subculture,and embryos of various developing stages occured. Embryos could germinateinto plantlets.When transferred onto germination medium. The plantlets of Lumian 6,andcotyledonous embryos of Coker 201 and Siokra 1~3were obtained in this experiment.
【关键词】 陆地棉;
花药愈伤组织;
胚胎发生;
植株再生;
【Key words】 Gossypium hirsutum L.; anther callus; embryogenesis; plant regeneration;
【Key words】 Gossypium hirsutum L.; anther callus; embryogenesis; plant regeneration;
【基金】 国家自然科学基金
- 【文献出处】 江西农业大学学报 ,ACTA AGRICULTURAE UNIVERSITIS JIANGXIENSIS , 编辑部邮箱 ,1994年04期
- 【分类号】S562.035.3
- 【被引频次】7
- 【下载频次】172