【摘要】 将鸡马立克氏病毒（ＭＤＶ）血清２型Ｚ＿４毒株第１０代（Ｚ＿４－１０），在对鸡马立克氏病（ＭＤ）高度易感的Ｐ系ＳＰＦ鸡胚成纤维细胞（ＣＥＦ）单层上连续传代全第３０代（Ｚ＿４－３０），Ｚ＿４毒株空斑形成速度和空斑形态均未发生显著变化。分别以Ｚ＿４－１１，Ｚ＿４－２０，Ｚ＿４－２５和Ｚ＿４－３０细胞结合毒作单价疫苗，将Ｚ＿４－１１，Ｚ＿４－２０和Ｚ＿４－３０细胞结合毒分别与Ｆ＿（Ｃ１２６）细胞结合毒组成二价疫苗，免疫Ｐ系ＳＰＦ鸡，用ＭＤＶ强毒ＧＡ株攻击，进行免疫效力试验。结果表明，Ｚ＿４毒株４个代次单价苗之间以及３个代次毒分别与Ｆ＿（Ｃ１２６）毒株组成的二价疫苗之间免疫效力无显著差异。表明Ｚ＿４毒株在ＣＥＦ单层上从第１０代连续传至第３０代，仍然保持其原有的培养特性和免疫原性。更多还原

【Abstract】 Different passages of Z₄ strain in chicken embryo fibrablast（CEF）culture were tested to evalu-ated the stability of characteristics in culture and immunogenicity of the virus Z₄ strain of serotype 2 MDV,Z₄strain was serially passaged from passage 11 to passage 30 in CEF nomolayer cuIture originated form line P（B19/B19） SPF chickens which are highly susceptible to Marek’s disease（MD）.The time interval requiredfor developing plagues, the average size and morphology of plaques, in defined period of time post-inocula-tion did not change siginificantly for Z₄ strain up to passage30.Protective efficiencies of four Z₄ monovalentvaccine, F_C126 monovalent vaccine and bivalent vaccines respectively containing passage 11 of Z₄ and F_C126, pas-sage 20 of Z₄ and F_C126 or passage 30 of Z₄ and F_C126 were compared, and the results showed that immunogenic-ity of Z₄ strain remained unchange up to passage 30.更多还原