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异体神经移植后再生神经的荧光逆行标记

Nerve regeneration through nerve homograft was trunsposed with a methoc of fluorescence labelling technige

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【作者】 曾志诚帅建中任力锋谢嘉平刘喜林谭维

【Author】 Zeng zhicheng,Shuai jianzhong.(Department of the Anatomy,Hunan Medical University)Ren Lifeng,Xie jiaping,Liu xilin.Tan wei.(Depatment of the MedicalEngimering, HunanMedical University)

【机构】 湖南医科大学人体解剖学教研室湖南医科大学神经生物学研究室湖南医科大学医学工程研究室湖南医科大学医学工程研究室 湖南长沙 410078湖南长沙 410078湖南长沙 410078

【摘要】 本文用WI ST AR大鼠作为实验模型,切除1cm坐骨神经,再用同系WI ST AR大鼠坐骨神经行异体桥接,修复坐骨神经的缺损。用FLURO—GO LD(F、G)或FAST、BLUE(F、B)两种荧光标记物,进行示踪检验,证实WISTAR鼠异体神经桥接能修复周围神经的缺损。

【Abstract】 In the experiment,the sciatic nerve of Wistar rats were cut off 1cm, and then the gap was bridged with nerve homograft of Wistar rats,two kinds of fluorochrome: fluor - gold (FG) and Fastblue(FB) were used in tracing the continuity of thecut sciaticnerve. the results demonstrated that the retrograde fluorescent tracing of FG and FB was a labelling method to detdct the regeneration of peripheral nerve fibres and the nerve bridge is a suitable bridging material for peripheral nerve reconstruction.

  • 【文献出处】 局解手术学杂志 ,Journal of Regional Anatomy and Operative Surgery , 编辑部邮箱 ,1994年01期
  • 【分类号】R322
  • 【下载频次】19
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