Correlation Between Unfolded States of Apocytochrome c and Its Ability to Pass Lipid Bilayer

【摘要】 <正> In contrast to the horse heart apocytochrome c,the chicken heart apocytochrome c under-went a conformational change from random coil to partial folding during a renaturation process.When theapocytochrome horse heart and that of chicken heart c were subjected to a translocation assay in vitro usinglarge trypsin-enclosed unilamellar vesicles from soybean phospholipids,the ability of the chicken heart apoc-ytochrome c to penetrate into the liposomes was found to decrease markedly with the renaturation procedure,while that of horse heart apocytochrome c remained relatively constant.Observations from circular dichroismmeasurement on the induction of secondary folding of these two species of apocytochrome c upon interactionwith soybean phospholipid vesicles suggested that a more flexible structure of apocytochrome c embedded inthe lipid matrix be required for its efficient translocation across the bilayer.更多还原

【Abstract】 In contrast to the horse heart apocytochrome c,the chicken heart apocytochrome c under- went a conformational change from random coil to partial folding during a renaturation process.When the apocytochrome horse heart and that of chicken heart c were subjected to a translocation assay in vitro using large trypsin-enclosed unilamellar vesicles from soybean phospholipids,the ability of the chicken heart apoc- ytochrome c to penetrate into the liposomes was found to decrease markedly with the renaturation procedure, while that of horse heart apocytochrome c remained relatively constant.Observations from circular dichroism measurement on the induction of secondary folding of these two species of apocytochrome c upon interaction with soybean phospholipid vesicles suggested that a more flexible structure of apocytochrome c embedded in the lipid matrix be required for its efficient translocation across the bilayer.更多还原