【摘要】 用胶原酶消化分离小牛肾上腺髓质嗜铬细胞，１０－４ｍｏｌ／Ｌ乙酰胆碱（Ａｃｈ）促进细胞儿茶酚胺的分泌，其作用具有Ｃａ２＋依赖性，回归方程ｒ＝－３．２９０＋５．２７５ｘ，相关系数Ｒ＝０．９９１０＞＞α０．０１＝０．７９８０．高Ｋ＋（６５ｍｍｏｌ／Ｌ）使膜去极化，也促进细胞儿茶酚胺的分泌，在一定范围内与Ｃａ２＋浓度呈正相关，回归方程ｙ＝１．３７６＋５．３３７ｘ，相关系数Ｒ＝０．９８５６＞＞α０．０１＝０．７９８１．磷酸川芎嗪（ＴＭＰ）浓度为１０－４～１０－７ｍｏｌ／Ｌ时，能抑制Ａｃｈ促进的分泌作用，１０－３～１０－７ｍｏｌ／Ｌ时能抑制高Ｋ＋促进的分泌作用．更多还原

【Abstract】 Calf adrenal chromaffin cells were isolated by removal of the cortex andsquential collagenase digestion of the medulla. After a short time cultivation inBuffer Ⅱ containing 5mg/mL bovine serum ablumin, Chromaffin cells were obtained in high yield and more than 95% of these were visible as shown by exclusionof Trypan Blue. The acetylcholine (10-4 mol/L) - stimulated release of catecholamine was dependent on the extracellular Ca2+ concentration,the regression equation was Y=-3. 290 + 5. 275X., correlation coefficient R =0. 9910>>α0.01 = 0.7980. The excess potassium (65mmol/L) -stimulated release was also correlativewith extracellular Ca2+ concentration, the regression equation was Y = 1. 376 + 5.337X,correlation coefficient R= 0. 9856>> α0.01 = 0’ 7980. The ligustrazine appearedto inhibit acetylcholineevoked release at oncentrions between 10-3 and 10-7mol/L,italso inhibited excess potassium-evoked release at the concentrations between 10-3and 10-7mol/L. But ligustrazine at/lower than the concentration of 10-8mol/L hadno effect on Ach or excess potassium-evoked release. It also showed that the ligustrazine might be poisonous to the chromaffin cells at high concentration (10-2mol/L).更多还原