【摘要】 为了观察冷冻速率和低温程度对组织细胞的损伤作用，将培养的大鼠主动脉内皮细胞（ＶＥＣ）在不加任何低温保护剂的情况下，置于速率冷冻装置内，以１℃／ｍｉｎ速率使培养液的温度分别降至０、－１０、－２０、－４０℃或以１０℃／ｍｉｎ速率降至－４０℃。在冻后１ｄ和３ｄ分别观察正常培养组和各冷冻组的细胞生长情况、培养液中乳酸脱氢酶（ＬＤＨ）活性及ＶＥＣ的因子Ⅷ相抗原（ⅧＲ．Ａｇ）免疫荧光反应的情况。结果可见，冷冻后ＶＥＣ的细胞数和生长率降低；ＬＤＨ活性增加；各冷冻组的ＶＥＣⅧＲ：Ａｇ免疫荧光细胞数和阳性率降低。在同一冷冻速率下，上述这些指标的改变随冷冻温度的降低而加重；而在同一冷冻温度下，冷冻速率愈慢，损伤程度愈重。更多还原

【Abstract】 In this study,the cultured vascular endothelial cells( VEC )of rat’s aorta were frozen at different temperatures. The number ,growth rate of VEC ,lactic dehydroge-. nase(LDH )activity and the Factor Ⅷ related antigen(ⅧR:Ag )in cultured medium were determined on the 1st day and the 3rd day after cooling.The results showed that the number and growth rate of VEC decreased ,LDH activity increased ,the number and positive rate of cells with immunofluorescence reduced after cooling ,and these changes were closely related to the final temperature and the cooling rate. It indicated that VEC were injured by low temperature even at 0℃in the absence of cryoprotector. The slower the cooling rate and the lower the final temperature ,the more severe injuries were induced.更多还原