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钝顶螺旋藻质粒的电镜观察及杂交研究
TEM OBSERVATION AND HYBRIDIZATION STUDY OF PLASMID FROM SPIRULINA PLATENSIS
【摘要】 于1992年6月-1993年4月,对纯项螺旋藻质粒进行透射电镜观察及分子杂交研究。结果表明,利用单层分子膜展开技术,能够观察到超螺旋以及闭合环形的质粒图象;S6质粒大小的平均值约为2.0kb,与电泳检测的结果稍有出入。斑点杂交结果表明,不同形态藻株S6、F3、F3弯与F3直质位之间具有一定同源性,与染色体DNA之间均显示同源性。对钝顶螺旋藻质位的进一步研究将推动其基因工程育种的开展。
【Abstract】 Transmission electron microscopic observation and hybridization study of the Plasmid from Spirulina platensis were carried out from June, 1992 to April, 1993.Supercoiled and closed circular patterns were observed under a transmission electron Wicroscope. The size of the plasmid from S6 strain was about 2.0kb, which Was slightly different from the result of electrophoresis. Results of dot blotting show that plasmids from strains of S6, F3, F3 spiral and F3 stfaight differing in form are komologous to each other and are also homologous to chromosomal DNAs.
- 【文献出处】 海洋与湖沼 ,Oceanologia Et Limnologia Sinica , 编辑部邮箱 ,1994年05期
- 【分类号】Q78
- 【被引频次】31
- 【下载频次】180