【摘要】 我们首次以键合有抗体的多孔玻璃作为固相免疫分析的免疫反应器,以化学发光作为最终检测手段,建立了一种新的、高效率的免疫分析技术—流动注射化学发光免疫分析技术.实验表明:采用该技术可使单次测定时间从ELISA(Enzyme-linked Immunosorbent Assay)法的二十多小时降至二十分钟,且所有操作均可在微机控制下自动完成.用该方法对人血清中乙型肝炎表面抗原的测定结果与ELISA法所得结果一致,对同一样品连续九次测定的相对标准偏差为7.2%. 因此,该方法具有自动化程度高、分析速度快、稳定性好等优点.更多还原

【Abstract】 In this paper, a new immunoassay technique, flow Ohemiluminescence injection immunoassay, in which controlled pore glass immobilized with antibody was used as immunoreactor and chemiluminescence for end-point monitoring, has been developed. Researches show that the time required per assay only 20 min with this method while 20 h with conventional ELISA. Moreover, the whole assay can been taken automatically under the control of a microprocessor. This technique has been used for the determination of HBsAg in serum, and the results are comparable with that of ELISA. In addition, it has a relative standard deviation of 7.2 % (9 assays). So this technique offers advantages of automatically rapidly and stablity.更多还原