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稳定提高糖化酶生产酶活力的研究

STUDY ON THE STEADY INCREASE OF ENZYMATIC ACTIVITY OF GLUCOAMYLASE IN INDUSTRY

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【作者】 安志法王松岭张爱清孙军

【Author】 An Zhifa; Wang Songling; Zhang Aiqing; Sun Jun(Henan Institute of Biology,Zhengzhou,450003)

【机构】 河南省科学院生物研究所

【摘要】 对糖化酶在工业生产中影响酶活力的几个主要因素进行了试验研究,获得培养基最适浓度为20.25%,准确控制发酵培养基的标准体积、浓度及其适宜通风量(前期控制在1:0.40~0.47,中期为1:0.55~0.65,后期为1:0.5~0.55)是糖化酶生产中稳产高产的关键技术措施。结果表明:采取该措施提高酶活力47.32%,经济效益显著。

【Abstract】 ABSTRACT Through experiment and research on several key factors affacting the enzymatic activity ofGlucoamylase in industry,we discovery that the most appropriate concentration of the mediumis 20.25%,and that controlling strictly standard volume and concentration of fermentation me-dium and appropriate ventilation(early stage 1:0.40—0.47.middle stage 1:0.55—0.65,laterstage 1:0.50—0.55.)is the key technological measures to ensure high and stable yields ofGlucoamylase.The testing results show that adopting the nieasures can increase the enzymaticactivity by 47.32% with notable economical gainings. Key wards Glucoamylase,Fermentation medium

【关键词】 糖化酶发酵培养基
  • 【分类号】TQ925
  • 【被引频次】1
  • 【下载频次】180
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