【摘要】 利用鼠鼠杂交瘤２Ｆ＿７细胞（分泌ＩｇＧ＿２单抗），研究了普兰卢内克（Ｉ）、聚乙二醇（ＩＩ）、甲基纤维素（ＩＩＩ）、葡聚糖（ＩＶ）、聚醚多元醇（Ｖ）与杂交瘤细胞的生物相容性、添加限制浓度以及高速搅拌下添加剂对杂交瘤细胞的保护作用。实验结果表明，０．０５％～０．１％的Ｉ、０．０４％～０．２０％ＩＩ、０．１０％～０．２０％的ＩＩＩ能较好地保护杂交瘤细胞；ＩＶ会抑制细胞生长；ｖ会分解细胞。在１．５Ｌ生物反应器中培养，添加０．１０％Ｉ于培养基，搅拌转速为７０ｒ／ｍｉｎ时，细胞能正常生长。更多还原

【Abstract】 The effects of different protective agents such as pluronic F-68,polyethyleneglycOl （PEG）,methylcellulose（MC）,dextran, on hybridoma 2 F₇ cell culture which secretesmonoolonal antibody against small cell lung cancer were studied. The potential toxicity,restricting adding concentration, protective effeccs at high agitation speed were investigated. It is shown that 0.05%～0.1%pluronic F-68,0.04%～0.2%PEG,0.1～0.2%MC canprotect hybrdoma cell from shear stress at high agitation-speed,dextran inhibits cell growth. In 1.5 L celligen bioreactor, adding 0.1% pluronic F-68 in medium,at 70 r/min of agitatingspeed,the hybridoma cells can normally grow.更多还原