By the methed of two steps of enzymes hydrolysis, the protoplasts were prepared from etiolated maize leaves. It was found that for the first step of enzymes hydnolysis,it was suitable that leaves were incubated with cellulase(0. 7%) and pectinase (0. 5% ) for 1 hour at 30℃, for the second step of enzymes hydrolysis, the suitable incubation duration was 2. 5 to 3 hours at 30℃ in above enzymes solution, It was also found that the purified protoplasts could be obtaind officiently from the supernatant through s...
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