【摘要】 应用双相电泳技术对冬小麦干旱诱导蛋白研究结果表明,10×10^-6脱落酸（ABA）对于抗旱性强的冬小麦太原633和抗旱性弱的C609干旱诱导蛋白的形成没有作用,100×10^-6ABA可以使太原633产生分子量为17.5～20KD,PI4.7～5.5的一些新蛋白;50×10^-6和100×10^-6ABA能诱导C609形成15～17.5KD,PI5.2～5.7的几个新蛋白和一个26KD、PI5.8的新蛋白。而在干旱胁迫下,太原633产生的一些诱导蛋白为17.5、20KD,PI4.7～5.5,C609仅产生26KD、PI5.8的新蛋白。由此表明,不同浓度的ABA对冬小麦干旱诱导蛋白的效应是不同的,且与抗旱性有关;ABA和干旱胁迫对干旱诱导蛋白的形成有一定的差异性。更多还原

【Abstract】 The results of study conducted by use of two-dimensional gel electrophoresis show that 10×10-6 ABA has no effect on induction of drought-induced proteins in drought-resistant winter wheat Taiyuan633 and drought-sensitive winter wheat C609. A group of new proteins with 17. 5-20 KD and PI values of 4. 7-5.5 can be induced with treatments of 100 × 10-6 ABA in Taiyuan 633, and several new proteins with 15- 17. 5 KD and PI values of 5. 2 - 5.7, and a 26 KD protein with PI value of 5. 8 can be induced with treatment of 50×10-6 or 100 × 10-6ABA in C609. But under drought stress a group of new proteins with 17.5-20KD (PI4. 7-5. 5) can be induced in Taiyuan633. only a 26KD protein (PI 5. 8) in C609. This shows that effects of different concentration of ABA on drought-induced proteins in winter-wheat are different, moreover, related to plant drought resistance. Meanwhile. there are differences in induction of drought-induced proteins between ABA and drought stress.更多还原