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软紫草愈伤组织的初步培养
PRELIMINARY STUDY ON CALLUS CULTURE OF ARNEBIA EUCHROMA
【摘要】 外植体来源不同的软紫草愈伤组织产生紫草色素的能力各异。60Co-r射线辐照处理后的H2无性系愈伤组织生长量和色素产生均属上乘。改良MS基本培养基添加1毫克/升KT,0.5毫克/升IAA,5%(W/V)蔗糖对愈伤组织培养较为适宜。马铃薯提取液对愈伤组织生长有明显的促进作用。
【Abstract】 Callus of various explants of Arnebia euchroma had different ability to produce shikonin. H2 clone which was treated by 60Co-r rays had higher production in both callus yield and shikonin than the others. A modified MS medium with 1 mg/l KT, 0.5 mg/l IAA, 5% sucrose stimulated production of callus and shikonin. Potato extract was benefit to callus production.
- 【文献出处】 广西植物 ,GUIHAIA , 编辑部邮箱 ,1994年04期
- 【分类号】Q949.777.4
- 【被引频次】12
- 【下载频次】188