【摘要】 用免疫印迹技术（IBT）检测了正常人及病人124份血清中的盐水可提取核抗原（ENA）,结果表明在各类风湿性疾病中,抗Sm抗体主要见于系统性红斑狼疮（SLE）,阳性率为23.5±10.5％,抗U₁RNP抗体主要见于混合性结缔组织病（MCTD）,阳性率为92.3±7.4％。高于在SLE中的阳性率17.6±9.2％,具有极显著差异性（P<0.01）;抗SSA抗体主要见于干燥综合征（PSS）,阳性率为75.0±21.7％;抗SSB抗体亦主要见于干燥综合征,阳性率为75.0±21.7％,明显高于在SLE中的阳性率11.8±7.8％及MCTD中的阳性率7.7±7.4％,具有极显著性差异（P<0.01）;抗Scl-70抗体则特异性地见于进行性系统性硬化症（PSS）,阳性率为33.34±27.2％;抗Jo-1抗体特异性地见于多发性肌炎（PM）和皮肌炎（DM）,阳性率25.04-21.7％;抗双链DNA抗体（ds-DNA抗体）亦特异性地见于SLE,阳性率为11.8±7.8％,而正常人及非风湿性疾病患者则为阴性。因之,测定血清中ENA多肽抗体中的7项多肽抗体对诊断SLE、MCTD、SS、PSS和多发性肌炎、皮肌炎等病是比较有意义的指标。更多还原

【Abstract】 Extractable nuclear antigen (ENA) in serum of 124 ordinary person and patient was detected by immunoblotting technique (IBT). The result indicated that in various rheumatism anti Sm antibody was mainly seen in systemic lupus erythematous (SLE), its positive rate’was 23. 5±10. 5%; that anti V. RNP antibody was seen in mixed connective tissue disease (MCTD) and its positive rate was 92. 3±7. 4% and higher than positive rate in SLE (17. 6 ± 9.2%) and very obricus in difference (P< 0.01); that anti SS-A antibody was ween in sica syndrome (SS) and its positive rate was 75. 0±21. 7% ; that anti SS-B antibody was also mainly seen in SS, its positive rate was 75. 0±21. 7% and higher obviously than positive rate in SLE (11. 8±7. 8%) and in MCTD (7. 7±7. 4%) and very obvious in difference (r<0. 01); that anti scl-70 antibody was specially seen in progressive systemic sclerosis (PSS) and its positive rate was 33. 3±27. 2%; that anti J0-l antibody was specially seen in polymyositis (PM) and dermatomyositis (DM) and its positive rate was 25. 0±21. 7%; that anti ds-DNA antibody was also seen in SLE and its positive rate was 11. 8±7. 8%. The negative result was gained from other norheumatism and ordinary person. Because of those mentioned above, detecting 7 ENA polypeptide antibodies in serum may be regarded as significant index of diagnosing SLE, MCTD, SS, PSS, PM and DM.更多还原