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羟自由基（OH）、超氧阴离子（O2－）与线粒体低水平化学发光相关性的研究
STUDY ON CORRELATION BETWEEN OH,RADICALSAND LOW-LEVEL CHEMILUMINESCENCE FROMMITOCHONDRIA
【摘要】 以线粒体为模式体系，研究了低水平化学发光的过程和机制。实验发现在一定浓度范围（蛋白０－１００毫克／毫升）内，线粒体悬液的发光是随浓度的升高而增强。最适宜的测量条件为３５℃和ｐＨ＝７．Ａ。注入外源性ＯＨ和ＯＨ的清除剂证实，ＯＨ可起动脂质过氧化反应，且￣１Ｏ＿２是构成发光的体内光子源。超氧化物歧化酶（ＳＯＤ）对线粒体发光有调节作用。
【Abstract】 itochondria was used a sa model system to study the course and mechanism of low-level chemiluminescence. It is discovered that the LCL of mitochondria suspension riseswith its concentration increasing under the definited range （0-100 mg/mL protein）. Thebest satisfactory condition is 35℃ and PH=7.4. By adding of exogenous OH and OHcleaner, it is convicted that OH can initiate lipld peroxidation reactions and ￣1O2 is one ofthe main photon sources. SOD（in vivo） may adjust the LCL from mitochondria.
【基金】 国家自然科学基金
- 【文献出处】 感光科学与光化学 ,PHOTOGRAPHTC SCIENCE AND PHOTOCHEMISTRY , 编辑部邮箱 ,1994年04期
- 【分类号】O644.17
- 【被引频次】2
- 【下载频次】106