【摘要】 蛋白质与铁（Ⅲ）－铬天青Ｓ结合反应的研究魏永巨，李克安，童沈阳（北京大学化学系，北京，１００８７１）关键词血清白蛋白；γ－球蛋白；铁（Ⅲ）－铬天青Ｓ配合物；吸收光谱某些金属配合物与蛋白质的结合反应已被用于蛋白质的定量分析［１～４］。我们用吸收光谱法研...更多还原

【Abstract】 In pH 3.0 solution,proteins may react with Fe(Ⅲ)-chrome azurol S binary complex to form stable ternary complexes.The maximum absorption wavelengths of the binary and ternary complexes are 570 nm and 660 nm,respectively.According to the absorption spectra of this system,we consider that the combination is primarily due to electrostatic forces between protonated ammonium group of proteins and sulfonic acid group and carboxyl group of chrome azurol S.The sensitiveties(Sandell index)of detection for bovine serum albumin and human γ-globulin are 0.04and 0.16μg/cm2,respectively,which are about 15 to 35 times as high as that of the bromocresol green method.更多还原