【摘要】 本文以大肠杆菌为天冬氨酸脱氨反应的酶源，采用氨气敏电极为基础电极研制成Ｌ－天冬氨酸微生物电极。对细菌培养条件、固定方法及电极工作条件等进行了研究，测定Ｌ－天冬氨酸Ｅ～１ｇＣ曲线斜率为５５ｍＶ，线性范围为６．０×１０^－４～８．０×１０^－３ｍｏｌ／Ｌ，寿命可达１０天．更多还原

【Abstract】 An electrode for the determination of L-Aspartate was prepared.Escherichia coli was used as the biocatalyst and an ammonia gas electrode was used as the base electrode.The effects of the culture medium,immobilized methods of the bacteria and the response characteristics of the electrode were studied.The slope of the L-Aspartate response curve E～-IgC is 55 mV/decade,linear range is 6.0×10￣(-4)-8.0×10￣(-3)mol/L and life time is 10days.更多还原