【摘要】 应用单克隆抗体对广东省１９８５～１９９２年脊髓灰质炎（下称脊灰）流行区的急性迟缓性麻痹（ＡＦＰ）病例粪便标本中分离出的２５４株脊灰病毒作病原分析．结果Ⅱ、Ⅲ型均未发现野毒株，而Ⅰ型２０５株中有１１９株为野毒株，７１株的抗原排列具野毒株和疫苗株的双重抗原位点，且有逐年增加的趋势，表明此期间广东的脊灰流行株主要为Ⅰ型。抽取具双重抗原位点毒株中的２株同时用Ⅰ型野毒株及ＳａｂｉｎⅠ疫苗株的特异ＰＣＲ检测，并进行核甘酸测序，发现其基因组在３２６２／３２６３处重组，证实为后二者的重组株，此２例患者均有服苗史，早期检测有高滴度抗体，提示其可能成为消灭脊灰不可忽视的障碍。更多还原

【Abstract】 Monoconal antibody panels were applied to identify 254 Poliovirus samples isolated from cases of AFP (acute flaccid paralysis) from Guangdong province,1985-1992.None of type Ⅱ and type Ⅲ is wild strain.of 205 strains of type Ⅰ,119 are wild,71 have both wild and vaccine strainantigenic sites,the later showed trend of yearly increase.It shows that typeⅠis prevalent strain.By PCR and sequence analysis,two strains of type I with wild and vaccine antigenic phenotypeswere confirmed as recombinant of wild and vaccineviruses.The recombinant site is shown in genome3262/3263,From the epidemiogy of the two cases,the recombinant virus seems to be a new obstacle for the eradication of Poliomyelitis.更多还原