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对流免疫电泳检查不同材料中的法氏囊病毒抗原
DETECTION OF INFECTIONS BURSA DISEASE VIRUS ANTIGENS IN DIFFERENT MATERIALS BY COUNTER IMMUNO-ELECTROPHORESIS
【摘要】 采用琼脂扩散试验(AGD)和对流免疫电泳(CIE)两种方法对比检查了不同材料中的传染性法氏囊病毒抗原,对感染鸡的法氏囊组织,CIE检查病毒抗原的敏感性较AGP提高了4~8倍;对病毒含量少,AGP不能检出的鸡胚材料,CIE仍能从鸡胚胚体和绒毛尿囊膜中检出病毒抗原,其检出率为9.1%~27.2%。
【Abstract】 Comparison had been made to detect infectious bursa disease virus antigens in different materials by agar gei precipitation (AGP) and counter immuno-electroph-oresis(CIE).CIE was four to eight times sensitive as AGP to detect virus antigens in bursa tissues of affected chickens.As to embryo materials which had a very low virus content and AGP was not sensitive enough to detect it, CIE still could iden- tify virus antigens in embryo bodies and chorioallantoic membranes.The positive rates ranged from 9.1 to 27.2 percent.
【关键词】 对流免疫电泳;
绒毛尿囊膜;
鸡胚;
抗原;
【Key words】 counter immuno-electrophoresis; chorioallantoic membrane; embryo; antigen;
【Key words】 counter immuno-electrophoresis; chorioallantoic membrane; embryo; antigen;
- 【文献出处】 贵州农业科学 ,GUIZHOU AGRICULTURAL SCIENCES , 编辑部邮箱 ,1994年02期
- 【分类号】S858.315.3
- 【被引频次】4
- 【下载频次】30