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对流免疫电泳检查不同材料中的法氏囊病毒抗原

DETECTION OF INFECTIONS BURSA DISEASE VIRUS ANTIGENS IN DIFFERENT MATERIALS BY COUNTER IMMUNO-ELECTROPHORESIS

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【作者】 李永明吴彤汪德生黄兵杨胜荣

【Author】 Li Yongming ;Wu Tong ;Wang Deshen ;Huang Bing ;Yang Shenyong(Department of Veterinary Science and Animal Husbandry,Guizhou Agricultural College, Cuiyang 550025)

【机构】 贵州农学院牧医系

【摘要】 采用琼脂扩散试验(AGD)和对流免疫电泳(CIE)两种方法对比检查了不同材料中的传染性法氏囊病毒抗原,对感染鸡的法氏囊组织,CIE检查病毒抗原的敏感性较AGP提高了4~8倍;对病毒含量少,AGP不能检出的鸡胚材料,CIE仍能从鸡胚胚体和绒毛尿囊膜中检出病毒抗原,其检出率为9.1%~27.2%。

【Abstract】 Comparison had been made to detect infectious bursa disease virus antigens in different materials by agar gei precipitation (AGP) and counter immuno-electroph-oresis(CIE).CIE was four to eight times sensitive as AGP to detect virus antigens in bursa tissues of affected chickens.As to embryo materials which had a very low virus content and AGP was not sensitive enough to detect it, CIE still could iden- tify virus antigens in embryo bodies and chorioallantoic membranes.The positive rates ranged from 9.1 to 27.2 percent.

  • 【文献出处】 贵州农业科学 ,GUIZHOU AGRICULTURAL SCIENCES , 编辑部邮箱 ,1994年02期
  • 【分类号】S858.315.3
  • 【被引频次】4
  • 【下载频次】30
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