A new kinetic fluorimetric method has been proposed for the determination of trace iron. The method is based on the catalytic effect of iron (Ⅱ) in cooperation with tetraethylenepentamine on the oxidation of reduced dichlorofluorescein with hydrogen per oxide. The linear range of the method is 0. 01 - 0. 30 μ/25 ml. The detection limit is 1. 3×10-10 g/ml. There is no interference from most common ions. The method has been used to determine trace iron in hair, human serum, fingernail and wheat flour, The rec...