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单糖的高效毛细管电泳间接紫外检测法研究
Indirect Ultraviolet Detection of Monosaccharides by High-performance Capillary Electrophoresis Using β-Indoleacetic Acid-Phosphate as Background Electrolyte
【摘要】 本文研究了单糖的毛细管电泳间接紫外检测法。实验发现选用β-吲哚乙酸-磷酸钠体系作为背景电解质比较理想,用于9种未经衍生的单糖进行毛细管电泳分离和间接紫外检测可取得满意结果.对半乳糖、甘露糖和葡萄糖酸的检出极限为2～10pmol水平.本文还讨论了背景电解质浓度与样品响应值的关系以及各种操作条件的影响.
【Abstract】 Indirect UV detection of monosaccharidcs by capillary electrophoresis was investigated. The introduction ofβ-indoleacetic acid-phosphate system as an ideal background elec-trolyte allowed sensitive indirect UV detection of underivatized monosaccharidcs. Under optimum condition, a detection limit of 2 -10 pmol level was achieved for galactose, mannose and gluconic acid. The effects of composition and concentration of the background electrolyte on the response value of sample etc. were also discussed.
【关键词】 单糖；
毛细管电泳；
间接紫外检测；
β-吲哚乙酸-磷酸盐；
【Key words】 Monosaccharides； Capillary electrophoresis； Indirect ultraviolet detection. β-In- doleacetic acid-phosphate system.；
【Key words】 Monosaccharides； Capillary electrophoresis； Indirect ultraviolet detection. β-In- doleacetic acid-phosphate system.；
【基金】 国家自然科学基金;中国科学院化学研究所所长择优基金
- 【文献出处】 分析化学 ,Chinese Journal of Analytical Chemistry , 编辑部邮箱 ,1994年08期
- 【分类号】O658.9
- 【被引频次】15
- 【下载频次】238