【摘要】 本文采用ＰＣＲ技术对４例正常喉组织、１０例正常白细胞、３３例喉癌组织，进行Ｐ５３基因的５－６和７－８外显子检测，结果发现喉癌中Ｐ５３基因突变率为６９．６９％（２３／３３）（Ｐ＜０．０１），其中５－６外显子突变率为２４．２４％（８１３３），７８外显子突变率为４５．４５％（１５／３３）。各期喉癌Ｐ５３基因突变率无明显差异（Ｐ＞０．０５）。研究表明Ｐ５３基因的突变是喉癌发生发展过程中的一个重要机制。更多还原

【Abstract】 Abstract Mutation exon 5-8 of P53 gene in 33 surgical specimens of laryngeal carcinoma(LC)was in-vestigated using PCR technique. Our results suggested that P53 gene mutation rate in LC was 69. 69%(23/33)(P<0. 01), with exons 5-6 mutation reat 24.24%(8/33) and exons 7-8 mutation rate 45. 45%(15/33). The mutation rate did not correlated with different stage of LC(P>0.05).Our results proved that P53 gene mutations is an important mechanism in LC occur-rece and development.更多还原