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Ag-NOR电镜染色技术

Ag-NOR Staining Method at the Electron Micros-copical Level

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【作者】 陆珍凤周晓军张太和喻毅强

【Author】 Lu Zhenfeng Zhou Xiaojun Zhang Taihe Yu Yiqiang(Department of Ultrastructure Pathology,Jinling Hospital, Nanjing)

【机构】 南京军区总医院电镜室

【摘要】 Ag-NOR电镜染色技术陆珍凤,周晓军,张太和,喻毅强(南京军区总医院电镜室,南京210002)人胃癌组织的核仁组成区嗜银蛋白(Ag-NOR)可以应用一个既简单又可重复的新方法 ̄[1],在电镜水平下定位。此法是具有一个乙酰化过程的一步Ag-NOR染色...

【Abstract】 Silver stainable proteins of the Nucleolar Organizer Regions(Ag-NOR proteins ) of human stomach cancertissues have been localized at the electron microscopical level with a new method which combines a simple andreproducible one step Ag- NOR staining method combined with an acetylation procedure. This new method al-lows the fine identification of nucleolar components,particularly those which are stained by silver. The new method proposed here appears to be a useful tool for the simultaneous study of the localization of ribonucleoprotein and Ag-NOR proteins during the cell cycle.

  • 【文献出处】 电子显微学报 ,JOURNAL OF CHINESE ELECTRON MICROSCOPY SOCIETY , 编辑部邮箱 ,1994年02期
  • 【分类号】R735.2
  • 【被引频次】1
  • 【下载频次】34
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