【摘要】 本文用免疫组织化学方法和免疫电镜方法对１４只树脑β－内啡肽能神经元胞体和纤维的分布及其在细胞器的定位进行了研究。结果表明，本文首次报道在Ｂｒｏｃａ斜角带观察到β－内啡肽免疫反应阳性神经元胞体，电镜观察到β－内啡肽免疫反应物质定位于大颗粒囊泡内的小颗粒上和粗面内质网上。下丘脑弓状核及其附近区域观察到β－内啡肽免疫反应阳性神经元胞体。在室周区、室旁核、第３脑室室管膜下层及室管膜上皮细胞间、内侧基底下丘脑及其外侧区、正中隆起内带和外带部可见到β－内啡肽免疫反应阳性纤维和串珠状的膨体。对β－内啡肽的释放途径及其调节因素作了探讨。更多还原

【Abstract】 In the present`paper,immunohistochemical and immunoelectronic microscopic methods was used to research the distribution of the β-END neuronal perikarya and fibers in the brains of 14 Tupaia belangeri, as well as its the localization of the organellae,the resultshowed that immunoreactive neuronal perikarya of the β-END in the diagonal band of Broca were first reported in this paper.The electronic microscopic observation showed that the positive products of immunoreactivity to β-END antiserum are located at the small pellets within the large granular vesicles and scattered on the rough surfaced endoplasmic reticulum.Immunoreactive neurons perikarya to β-END antiserum in the hypothalamus arcuate nucleus and its surrounding areas were visualized by light microscopy.Immunoreactive positive fibers and beaded varicosities to β-END antiserum were found in the periventricle areas,the paraventricle nucleus,the vicinity of the ependymal layer of the third ventricle and ependymal surface,mediobasal hypothalamus and its lateral areas,and in the internal and external layer of the median eminence.The release ways and its regulative factors of the β-END are discussed in the paper.更多还原