【摘要】 将大白鼠肝脏切片和尿酸酶分别固定在不同氧电极上,制成尿酸组织电极和酶电极,研究了它们的测试条件、分析性能和应用情况.结果:组织电极的最佳测试条件为33℃,pH=9,Tris-HCl缓冲溶液,线性范围3.33～56.67μg/mL,K_M=3.3×10^-4mol/L;酶电极的最佳测试条件为28℃,pH=8.8,Na₂B₄O₇-（NH₄）₂SO₄缓冲溶液,线性范围6.67～133.3μg/mL,K_M=2.8×10^-4mol/L.二种电极测量值的变异系数均小于4%,电极性能各有优缺点.用它们测定质量控制血清,测量值均在示值范围内,有望应用于临床.更多还原

【Abstract】 An enzymic electrode and a tissue electrode were made by mounting uricaseand big mouse fresh liver onto the different oxygen e1ectrodes. The testing conditions,analytic properties and app1ications were measured and studied. The optilnal conditionsof the tissue electrode and that of enzymic electrode were respectively determined bel-low:33℃, pH= 9（Tris-HCl buffer）; linear range 3.3-56.67μg/mL, 28℃, pH = 8. 8（Na2B4O7-（NH4）2） SO4 buffer), linear range 6.67-133.3μg/mL. The CV of detectingvalues of the two electrodes all are less than 4% and the Michaelis constants of the en-zymic e1ectrode and tissue electrode were 2. 8×10^-4 mol/L and 3. 3 × 10^-4 mol/L respec-tively. The data of the measurments in detecting control serum with the two electrodeswere all in allowing range. With further study and development the two electrodes canbe used in clinic.更多还原