【摘要】 应用反转录－聚合酶链式反应（ＲｅｖｅｒｓｅＴｒａｎｓｃｒｉｐｔｉｏｎ一ＰｌｙｍｅｒａｓｅＣｈａｉｎＲｅａｃｔｉｏｎ，ＲＴ一ＰＣＲ）方法，亚克隆出大鼠小脑和肾脏ＮＯＳｃＤＮＡ，并以此为探针，通过Ｎｏｒｔｈｅｒｎｂｌｏｔｔｉｎｇ分析和定量ＰＣＲ方法，测定大鼠中枢和外周一些组织中ＮＯＳｍＲＮＡ的含量，证明ＮＯＳｍＲＮＡ广泛存在于中枢和外周组织中，在中枢以小脑含量为最高，在外周组织中以肾脏含量为最高；定量ＰＣＲ方法测定ＮＯＳｍＲＮＡ较Ｎｏｒｔｈｅｒｎｂｌｏｔｔｉｎｇ分析更为灵敏。更多还原

【Abstract】 With Reverse Transcription- Polymerase Chain Reaction(RT- PCR),nitric oxide synthase(NOS) com-plementary DNA has been subcloned from rat cerebellum and kidney.The levels of NOSmRNA in some cen-trical and peripheral tissues in rat have been detectd by Northern blotting and quantitative PCR.It has been proved that NOS mRNA is existed extensively in centrical and peripheral tissues.Among of them,NOS mR- NA Ievels are highest in cerebellum in centre and in kidney in peripheral tissues.Moreover, quantitative PCR is more sensitive than Northern blotting in detecting NOS gene expression.更多还原