【摘要】 经特异免疫沉淀法和２．２，１５细胞株的ＨＢｓＡｇ、ＨＢｅＡｇ的抑制实验，以阿糖腺苷为阳性对照，从１０４种中草药中优选出单味中药（９１２）对ＨＢＶ－ＤＮＡＰ、ＨＢｓＡｇ和ＨＢｅＡｇ抑制率高达９０％左右。９１２经大孔吸附树脂粗分成１１个部位，其中Ｂ、Ｇ、Ｈ、１４个部位经抗ＨＢＶ及抗氧自由基实验证明为活性部位。经定性与定量分析，Ｂ、Ｇ、Ｈ、Ｉ中富含黄酮及多元酚类（鞣质）成分，鞯质含量与其抗ＨＢＶ活性成正相关。更多还原

【Abstract】 We have tested 60 kinds of traditional Chinese herbs used in Clinical treatment of liver diseases,by in vitro HBV-DNAP inhibition test,We have also screened 44 kinds of medicines by the methodsof detecting the HBsAg and HBeAg recreted from the 2,2,15 cell line derived from the Hep-G2 celltransfected with cloned HBV-DNA. Ara-A was used as positive control drug. The one most activeChinese traditional drug found is“912”,its inhibition rates of HBV-DNAP、HBsAg and HBeAg are allover 90%. By means of DA201 and H103 Macroreticular resins,11 fractions were separated from 912,Four active fractions B、G、H、I were obtained by screening for Anti-HBV and Free-radical inhibition.By quatitative and quantitative analysis,fractions( B、G、H、 I)Found rich in flavonoids and polyphe-nol(tannins)constituents,Their activity on anti HBV were relatlve to their quantitative content of tannins.更多还原