【摘要】 将不同的白血病细胞株与正常骨髓细胞按比例混合，体外模拟人缓解期白血病骨髓。用染色体核型分析、流式细胞仪－ＤＮＡ（ＦＣＭ－ＤＮＡ）及ＦＣＭ－免疫标志等方法测定，证明该三项实验的灵敏度达０．５％。初步临床验证上述方法亦有一定的应用价值更多还原

【Abstract】 Two human leukemia cell lines(HL-60 and Nalm-6 cell lines) were mixed wih normal bone mar-row(NBM)making a serial dilution to simulate minimal residual leukemic bone marrow Each modelhas some special features Conventional techniques such as chromosome analysis,flow cytometry(FCM)DNA estimation and FCM-mmunoassay were used for detection and the sensitivity of each methodis ten times above the traditional morphology estimation。For clinical application,9 cases of acute le-ukemia have been studied before treatment and after complete remission The conclusion is that thesemethods can be used for clinical detection更多还原