【摘要】 应用基因工程技术构建了两种可表达丙型肝炎病毒Ｃ蛋白和ＮＳ３蛋白双表位嵌合抗原Ａ（Ｃ７５－ＮＳ３ａ）和Ｂ（ＮＳ３ａ－Ｃ７５）的质粒，并在大肠杆菌中进行了表达。采用经过盐析和离子交换层析纯化的表达产物进行Ｗｅｓｔｅｒｎ印迹、抗Ｃ和抗ＮＳ３双抗体夹心ＥＬＩＳＡ试验，并用以检测Ｃ或ＮＳ３表位单特异性血清及系列血清，结果表明Ａ或Ｂ抗原均同时具备Ｃ和ＮＳ３抗原的免疫反应性，而且两种抗原的性能基本相似。在ＨＣＶ抗体检测中这类抗原可望替代配伍的Ｃ＋Ｃ３３ｃ混合抗原。更多还原

【Abstract】 wo plasmids,which express chimeric recombinant antigens A(C75-C33c)and B(C33c-C75),was constructed and expressed in E. coli.The chimeric antigens were salted out and purified through ion-exchange chromatography.The immunoreactivity was tested by Western blotting,sandwich ELISA and indirect ELISA. The results indicated that the capsid and NS3 epitopes on either antigen A or B were both immunoreactive.These chimeric antigens are expected to substitute currently used mixed antigens C+C33c in HCV immunoassay.更多还原