【摘要】 本文应用间接免疫荧光技术，研究无细胞毒性浓度的Ｇｅ－１３２在试管内对Ｅｐｓｔｅｉｎ－Ｂａｒｒ病毒（ＥＢＶ）抗原表达的影响。结果表明Ｇｅ－１３２明显地抑制ＥＢＶ相关抗原的表达，抑制程度随药物浓度增加而加强。５０～２００μｇ／ｍｌＧｅ－１３２对Ｂ９５－８细胞的ＶＣＡ、ＭＡ抗原抑制率为３９．２４－５２．２７％和１５．４２～３４．１５％，５－４０μ／ｍｌＧｅ－１３２共培育和预处理Ｒａｊｉ细胞均能非常显著抑制致癌物巴豆油和正丁酸联合诱导ＥＡ抗原，抑制率分别是１１．２９－５４．２１％及３９．１５－８０．０９％。以上提示Ｇｅ－１３２具有明显抑制靶细胞中ＥＢＶ抗原表达的作用，有可能用于ＥＢＶ相关疾病的预防。更多还原

【Abstract】 The influences of Ge-132 on the expression of Epstein-Barr Vi-rus (EBV) antigens were studied in vitro by indirect immunofluorescence staining. Theresults showed that the expression of EBV antigens was significantly inhibited by non-non-cytotoxic doses of Ge-132 and the inhibition was dose-dependent. Inhibition rateof Ge-l32 (50～200μg/ml) on VCA and MA expression by B95-8 cells were 39.24～52.27%and 15.42-34.1 5%respectively. Co-cultured and pretreated Raji cells with theconcentration of 5～40μg/ml of Ge-132 markedly inhibited the exoression of EA inducedby croton oil and n-butyric acid, inhibition rate were 11.29% to 54.21%and 39.15～80.08%.respecti vely. These results suggest that Ge-132 markedly inhibit the expression of EBVantigens in target cells, it may be used in the prevention of EBV-related disease.更多还原