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制备小鼠雄性生殖细胞微核标本的简易方法
A SIMPLE METHOD PREPARING SAMPLE FOR OBSERVING MlCRONUCLEUS IN MALE REPRODUCTIVE CELL5
【摘要】 本文报道了一种小鼠精细胞微核标本制备的简易方法,即将曲细精管剪碎,与适量的2.2%柠檬酸钠溶液混和后,直接取细胞悬液推片,甲醇固定后再用pH7.0的1/15M磷酸缓冲液处理,Ciemsa染色。
【Abstract】 A simple method for making preparation of samples to detect micronucleus in male mouse reproductive cells was described.Seminiferous tabules were taken from decoated testis and cut into fine pieces with ophthalmological scissors,mixed with a couple of drops of 2.2%Sodium Citrate Solution on a slide. Making smear with the aliquot.Fixed with methanol Stained with pH7.0 phosphate buffered Giemsa Dye Solution.Obseved unde micoscope. Pictures from this preparation were shown.
- 【文献出处】 癌变.畸变.突变 ,CARCINOGENESIS, TERATOGENESIS AND MUTAGENESIS , 编辑部邮箱 ,1994年06期
- 【分类号】Q2-33
- 【被引频次】12
- 【下载频次】47