【摘要】 免疫电镜、中和抑制试验和D-Ag检测证实,经甲醛处理灭活的PV-1/PV-2(XF414株)及PV-1/PV-3(XF3株)型间嵌合病毒株仍具有二价抗原性,用其免疫家兔,仍能诱导机体产生二价抗PV中和抗体.更多还原

【Abstract】 Purified viruses were inactivated with 1:4000 formalin at 37℃ for 12d. Before and after inactivation,XF414 with anti-PV-1 and anti-PV-2, XF3 with anti-PV-1 and anti-PV-3 were observed by immunoelectron microscopy and the results were positive.Neutralization inhibition test and D-Ag unit detection were conducted, and the results were as follow: XF414 had type 1 D-Ag 15971 units/ml and type 2 D-Ag 15644 units/ml; XF3 had type 1 D-Ag 13314 units/ml and type 3 D-Ag 15971 units/ml. When rabbits were immunized with XF414 and XF3, they produced bivalent antiserum:i. e.anti-PV-1 and anti-PV-2 induced by XF414 and anti-PV-1 and anti-PV-3 induced by XF3.更多还原