【摘要】 博莱霉素可诱导发生肺损伤,肺泡巨噬细胞（AM）在该病理过程发生发展中起重要作用。由于博菜霉素可引起AM形态明显改变,细胞的分泌和吞噬等诸多功能活跃,推测博莱霉素可能引起细胞骨架的功能和结构发生改变。已有实验证明764-3对博莱霉素A₆（BLM A₆）刺激的细胞有保护作用,但其作用机制还不清楚。本实验目的是探讨博莱霉素对AM微管（MTs）和微丝（MF）分布和量的影响,并为764-3对肺损伤的防治提供实验依据。更多还原

【Abstract】 Using bleomycin A6 (BLM A6) to stimulate normal and 764-3 pretreated alveolar macrophages (AM) in vitro,the distributions of microtubules (MTs) and microfilaments (MFs) were examined with an immunofluorescence micros- cope.Fluorescent strength was determined with a spectrophotometer and fluorospectrophotometer, and the adhering and spreading rates were determined. The results showed that BLM A6-stimulated AM spread fully and MTs increased markedly,forming a thick network in the cytoplasm.Much punctate and linear fluorescence was visible beneath the plasma membrane,these fluorescing structures were MFs.In the experimental cells MT and MF fluorescence was stronger than that in the control group.However,most of the above changes were reduced in the 764-3 pretreatment group. These results suggest that BLM A6 may promote the assembly and regulate reorganization of MTs and MFs,while 764-3 can stabilize these structures.更多还原