【摘要】 发根农杆菌(Agrobacterium rhizogenes)R1600菌株感染绞股蓝(Gynostemma pentaphyllum(Thunb.)Makino)叶外植体10天后,在外植体切口处便出现毛状根,28天后,转化率高达94%。毛状根经Southern分析,证明确已被转化。毛状根生长速度很快,在无激素的MS培养基中悬浮培养20天,其生物量增加9倍,并无愈伤组织产生。毛状根中总皂甙含量约为自然根的2倍。在悬浮培养过程中,培养物向培养基中分泌一定量的皂甙。更多还原

【Abstract】 Authors report here the establishment of an efficient transformation system for Gynostemma pentaphyllum (Thunb. ) Makino using Agrobacterium rhizogenes Rl600. Haity roots appeared on leaf explants 10 days after inoculation with the bacteria . Frequency of the explants transformed by R1600 was up to 94%. Transformation was confirmed by Southern analysis. Biomass of hairy root cultures suspended in hormone-free MS medium increased 9 times after 20 days of incubati- on. There was no callus formation on the hairy roots during suspension culture. Saponin content in the hairy root cultures was about 2 times as much as in the natural roots, saponins of the hairy root cultures were also released into growth medium as well.更多还原