【摘要】 对甘肃省河西走廊不同生态型芦苇（Phragmites communis Trin.）幼苗质膜磷脂组成、总膜脂脂肪酸组成、膜结合酶、膜流动性以及膜相变温度进行了研究。结果表明,4种不同生态型芦苇的质膜均含有相同的6种磷脂和7种脂肪酸组分,但其配比差别显著。脂肪酸不饱和指数和膜流动性依盐化草甸芦苇、盐化草甸-沙丘过渡地带芦苇、沙丘芦苇和沼泽芦苇顺序减小。4种不同生境芦苇质膜在4—6℃低温区和20—28℃高温区均发生膜相的转变,其中高温区相变温度差异明显。质膜结合的Ca²⁺、Mn²⁺、Mg²⁺-ATP酶活性与膜脂脂肪酸不饱和指数和膜流动性呈正相关。结合生境分析,对应于沼泽芦苇,质膜膜脂中磷脂酰胆硷和双磷脂酰甘油含量的增加和脂肪酸不饱和指数显著加大,与芦苇耐盐性有密切关系,而质膜膜脂中磷脂酰甘油含量的增加和脂肪酸不饱和指数适度加大,则与芦苇耐旱性有关。更多还原

【Abstract】 Lipid phospholipid and fatty acid compositions, fluidity, phase transition temperatures and membrane-bound Ca²⁺ ,Mn²⁺,Mg²⁺-ATPase activities of plasmolemmas extracted from four reed Phragmites communis Trin. ecotypes in Hexi Corridor of Gansu province were investigated. The results showed that all plasmolemmas of the four reed ecotypes consisted of the same six phospho- lipid and seven fatty acid components, but their proportions in the plasmolemma lipids of various reed ecotypes were markedly different. Index of unsaturated fatty acid （IUFA）and fluidity of plasmolemmas increased in the sequence of swamp-,dune-, meadow-dune transitional zone-,salt meadow reeds. The plasmolemmas of all reed ecotypes showed phase transition in the two ranges of low-,high temperatures （4—6℃, 20—28℃）, their phase transition temperatures in the latter were markedly different, Plasmolemma-bound Ca²⁺ ,Mn²⁺,Mg²⁺-ATPase activity positively related to IUFA and fluidity. Combining the membrane properties with environments, in comparison with swamp reeds, the increases of phosphatidylcholine, cardiolipin and the significant rising of IUFA in plasmolemma lipids are closely responsible for reed salt-tolerance, whereas the increase of phosphatidylglycerol and the suitable rising of IUFA make a contribution to reed drought-tolerance.更多还原