【摘要】 采用垂直板式聚丙烯酰胺凝胶电泳,检测了红皮云杉(Picea koraiensis Nakai)雌配于体4种同工酶型。并以6个基因位点编码的4个酶系统,分析了我国东北林区红皮云杉4个自然群体的遗传多样性与遗传分化。群体的多态基因位点比例是0.50。群体的平均杂合性的视察值是0.316,与预期值吻合。每个基因位点发现的的等位基因数是2.50,有效等位基因平均数是1.71。对6个基因位点的基因多样性测定表明,群体间的分化占0.59％总的基因多样性99％以上产生在群体内。群体间的平均遗传距离是0.01。更多还原

【Abstract】 Vertical slab polyacrylamide gel electrophoresis was used to examine inheritance of four isoenzymes in megagametophytes of Picea koraiensis. Gene diversity and genetic differentiation in four natural populations of P. koraiensis in Northeastern China were analyzed at six loci coding for four enzyme systems. The proportion of loci polymorphism in population was 0.5. The aver- age expected heterozygosity of all samples was 0.314,and the average observed heterozygosity of all samples was 0.316. The average number of alleles detected per locus was 2.50. The effective number of alleles per locus was 1.71. Measurement of gene diversity for the six loci showed a 0.0059 significance of interpopulation differentiation. More than ninety-nine percent of the total gene diversity resided within population. The mean genetic distance over all pairs of P. koraiensis was 0.0110.更多还原