【摘要】 本文报道建立了一种新的实验技术——固相反向间接血凝(SP-RPHA)与固相反向间接血凝抑制试验(SP-RPHI),分别用于检测流行性出血热抗原和特异抗体。作者对实验条件进行了筛选对比,得到了最佳实验条件,并用此法检测31份出血热患者血清和17份患者尿液,经与荧光法和反向间接血凝、血凝抑制试验比较,表明SP-RPHA与SP-RPHI法重复性好,特异性、敏感性均高于上述常规方法。更多还原

【Abstract】 The methods of SP-RPHA and SP-RPHI were estab- lished and applied in the assasy of HFRS virus antigen and specific antibody respectively. The conditions for the test were studied and compared, and the most suitable conditions were used. Thirty-one serum samples and 17 urine samples from the patients with HFRS were analysied by these meth- ods, and their results were compared with those obtained by immunoflurescence, reverse indirect hemagglutination and hemagglutination inhibition methods. It was shown that the sensitivities of SP-RPHA and SP-RPHI are higher than those obtained by the above mentioned routine methods.更多还原