【摘要】 普通烟草（Nicotiana tabacum）和粉蓝烟草（Nglauca）的叶肉原生质体经高Ca⁺+,高pH的PEG溶液诱导融合,并再生出体细胞杂种植株。杂种植株中发现形态偏普通烟草,雌蕊发育正常,雄蕊发育异常,且融合双亲部分细胞质和细胞核的杂种株,将此株与普通烟草栽培品种（speight G-28 ♂）回交,回交1代（BC₁）、回交2代（BC₂）保持高度不育,回交3代（BC₃）、4代（BC₄）育性有一定的恢复,并出现育性分离。让恢复一定育性的株系自交,获得有一定育性的稳定株系,继续与其它普通烟草栽培品种回交（speight G-80 ♂,Speight G-140 ♂,Jingyehang ♂等）,恢复其不育特性,并稳定地遗传。用普通烟草栽培品种（Jingyehuang ♂）反交时,后代育性恢复正常。并相应对各世代生物学性状进行了研究分析。更多还原

【Abstract】 The alloplasmic somatic hybrid plant, which was fairly similar to N. tabacum in morphological feature with normal pistil and abnormal stamen and fusion parental partial nucleus and plasmids, was produced by inducing protoplast fusion between Ntabacum and N.glauca.The results of studies on the fertility of the hyhrid progenies showed that the plants of BC₁ and BC₂ remained male sterible, fertility restored and segeragation also appeared in BC₃ and BC₄,after backcrossed to tobacco cultivar （Speight G-28 ♂）and somme selections were performed, a genetically stable and fairly fertile strain was obtained, whose fertility inherited stably when backcrossed to tobacco cultivar（Speight G-28 ♂, Jingyeheang ♀）. the fertility of progeny restored normal. The results of studies on biological characteritic and disease resistance of every generation are also described.更多还原