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水稻品种广亲和基因等位关系的遗传分析

GENETIC ANALYSIS ON ALLELIC RELATIONSHIP OF WIDE COMPATIBILITY GENES AMONG SEVERAL WCV (Oryza sativa L.)

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【作者】 顾铭洪; 游艾青; 潘学彪;

【Author】 Gu Minghong You Aiqing Pan Xuebiao (Jiangsu Agricultural College, Yangzhou 225001)

【机构】 江苏农学院; 江苏农学院 扬州 22500l; 扬州 22500l; 扬州 22500l;

【摘要】 通过双列杂交研究了8个广亲和水稻品种广亲和基因的等位关系。研究表明:品种CalotoC、Cpslo-17、Ketan Nangka、02428和轮回422之间相互杂交F1代的花粉育性和小穗育性均达到正常育性水平。F2代分离不明显,推测这些品种间广亲和基因相互等位,品种02428和轮回422的广亲和性受S5位点的广亲和基因S5n控制。 广亲和品种Ketan Nangka与Aus 373或Dular杂交,F1花粉均出现部分不育现象。轮回422与Aus 373杂交,F1花粉和小穗育性均显著低于正常水平;与Dular杂交的F1花粉育性接近正常水平,但小穗育性显著偏低。以上组合F2代群体均出现明显的育性分离,可育株与部分不育株的分离符合7∶2的分离比,说明品种Aus 373和Dular的广亲和性在遗传上受与S5位点不等位基因的控制,表现配子体不育的遗传特点。在品种Aus 373和Dular分別与Calotoc、Cpslo-17、02428之间的杂种F1育性正常,F2代有少数半不育株出现。推测在这些组合中,杂种育性的表达除了受主基因控制以外,还有微效基因参与作用。

【Abstract】 In order to investigate the allelic relationship of wide compatibility genes, 8 WCVs, ie Calotoc, Ketan Nangka, 68-83, Lunhui 422, Cpslo-17, 02428, Aas 373 and Dular were collected and crossed in diallel mating design. For analysis of genetic constitutions of F1 gamets, the F1s of each cross were crossed to tester variety Nanjing 11 (indiea) and Akihikari (japonica). Pollen fertility and spikelet fertility were investigated for F1 F2 and TF1 (Fl of testing crosses) of each cross together with their parents. The main results were obtained as follows: The wide compatibility genes among variety Calotoc, Ketan Nangka, Lunhui422 and 02428 were allelie each other. The fertility of F1 of the varieties was normal and no significant segregation in fertility was found.On the other hand, partial sterili ty occurred in F1 of the crosses of vareity Ketan Nangkn and Lunhui 422 either crossed to variety Aus 373 or Dular. Fertility segregation was detected in F2 generations and the ratio of fertile plants to semisterile plants was close to 7:2. The sterility occurred in those crosses was supposed to be gametophytic sterile and the wide compatibility gene of variety Aus 373 or Dular was non-allelic to S5 locus, In other words, besides the gene of S5n, another major gene was possibly responsible for the compatibility of varieties Aus 373 and Dular.The fertility of F1 of varieties 02428, Cpslo-17, Calotoc either crossed to Aus 373 or Dular was normal. However fertility segregation could be found in F2 populations of the crosses in some extent. It is postulated that nonalielic genes of Wide Compatibility are responsible for the sterility in F2 and the minor genes of varieties 02428, Cpslo-17 and Calotoc with positive effect on increasing fertility plays important role in reducing the fertility segregation of F2.No sterility was found in the cross of varieties Dular and Aus 373 both in F1 and F2 generations, which meant that the compatibility gene between Aus 373 and Dular was allelic each other.

  • 【文献出处】 中国农业科学 ,Scientia Agricultura Sinica , 编辑部邮箱 ,1993年01期
  • 【被引频次】36
  • 【下载频次】83
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