【摘要】 采用葡聚糖氧化将胃癌单抗(McAb)与抗癌药物柔红霉素(DNR)和氨甲喋呤(MTX)同时交联(McAb-PAD<分别采用噻唑蓝活细胞染色法(MTT 法)及~3H-TR 掺入法检测交联物的细胞毒作用,结果表明两种检测方法敏感性无明显差异,并且证实文联物对肿瘤靶细胞具有较强的选择性杀伤效应。更多还原

【Abstract】 It is first time to use dextren T-40 oxidative method to conjugate anti-gastric cancer mono-colonal antibody(McAb)with anti-tumor medicines of daunorubicin(DNR)and methotrexate(MTX)together.Cytotoxicity of conjugates was measured by MTT method and ~3H-TdR incor-poration method respectively.Both sensitivity is similar.The results have showed that this conju-gate exhibited selective cytotoxicity on human gastric cancer cells in vitro.更多还原