【摘要】 本文利用MYO 小卫星DNA 探针进行了肿瘤DNA 指纹的初步研究。继之应用DNA 重组技术·由pUC19—MYO 质粒中获得MYO 小卫星DNA 探针片段,将其插入到具有RNA 聚合酶启动子的pGEM—4Z质粒的多克隆位点.从而构建了pGEM—4Z—MYO 亚克隆。为制备RNA 探针以提高DNA 指纹的敏感性,进而提高小卫星DNA 多态性的检出率奠定了基础。更多还原

【Abstract】 The initial study of the tumour DNA fingerprints using MYO minisatellite DNA probe wascarried out,and then,by means of DNA recombinant techniques,the fragment of MYO min-isatellite DNA probe obtained from plasmid pUC19-MYO was inserted into plasmid pGEM-4Z containing RNA polymerase promotor,thus a sub-clone refferred as pGEM-4Z-MYOwas constucted.That made an offer of the conditions of preparing RNA probe in order to in-cerase the sensitivities of DNA fingerprinting and laid a foundation for raised the efficiency of de-tecting the polymorphism of the minisatellite DNA.更多还原