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新肽类抗生素B-3543的研究——Ⅱ.发酵、分离、纯化及理化性质(英文)
STUDIES ON A NEW PEPTIDE ANTIBIOTIC B-3533 Ⅱ. FERMENTATION, ISOLATION, PURIFICATION AND PHYSICO-CHEMICAL PROPERTIES
【摘要】 采用棉籽饼粉培养基,发酵培养144小时,新抗生素B-3543的效价可达3000μg/ml,通过CAD-40柱, DEAE-Sephadex A-25柱和硅胶柱进行分离和纯化,可制得白色粉末状的B-3543纯品。根据该素的理化性质和氨基酸组成,将其鉴定为一种含有15种氨基酸的新肽类抗生素。
【Abstract】 The optimal medium for fermentation was pharmamedia medium.The antibiotic titer reached about 3,000μg/ml after 144 hours of fermentation. The white powder of antibiotic B-3543 was obtained after purification by column chroma-tography on CAD-40, DEAE-Sephadex A-25 and silica gel, according to the physico-chemical propsrties and constituent of amino acids of antibiotic B-3543, it is considered to be a new peptide antibiotic consisting of 15 kinds of amino acids.
【关键词】 新抗生素B-3543;
发酵;
分离;
纯化;
理化性质;
【Key words】 New antibiotic B-3543; Fermentation; Isolation; Purification; Physico-chemical properties;
【Key words】 New antibiotic B-3543; Fermentation; Isolation; Purification; Physico-chemical properties;
- 【文献出处】 中国抗生素杂志 ,Chinese Journal of Antibiotics , 编辑部邮箱 ,1993年02期
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