【摘要】 用广州血管圆线虫成虫切制成 4 μm厚的冰冻切片作抗原,用间接荧光抗体试验检测感染广州血管圆线虫大鼠血清的抗体,结果在感染后 2 wk的 50份鼠血清中,呈阳性反应46份,阳性率为 92.0％;在感染后 4 wk的 19份鼠血清中,呈阳性反应 19份,阳性率为 100％;对照的阴性血清及盐水均呈阴性反应。本法检出率高,且感染早期出现阳性反应,可作广州血管圆线虫病早期辅助诊断。更多还原

【Abstract】 Frozen section specimens with a thickness of 4 μm were made from adult worms of An-giostrongylus cantonensis in order to detect the serum antibodies in rats infected with An-giostrongylus cantonensis by indirect fluorescent antibody test (IFAT). The results showed that the positive rates of the serum samples 2 and 4 weeks after infection were 92. 0%(46/ 50) and l00% (19/19), respectively. The results of serum samples in healthy rats and normal saline (as control) were negative. This technique proved to have a highly detectable rate and the positive reaction might occur in early infection. It might be used for the early supplementary diagnosis of angiostrongyliasis.更多还原