【摘要】 对棘球蚴病患者用ABC-ELISA法检测CAb,ABC-夹心双抗体法和Pernice法分别检测CAg和CIC,阳性率分别为87.0％(40/46)、15.6％(7/45)和17.8％(8/45)。在检测CAb阴性的6例中CAg阳性者4例,表明CAb与CAg同时检测可提高阳性检出率达95.6％(43/45)。 CAb阴性病例主要是肺棘球蚴病,占83.3％(5/6)。CAg的检出率仅15.6％,但特异性高,未出现交叉反应和假阳性反应。检测CIC不但检出率不高,而且非特异性反应与检测CAb时相似。更多还原

【Abstract】 The Avidin-Biotin-peroxidase Complex (ABC) ELISA was used for detection of circulating antibody (CAb),the ABC-double antibody sandwich ELISA for detection of circulat- ing antigen(CAg)and the method of Pernice’s for detection of specific circulating immune com- plexes (CIC)in sera of the patients with unilocular hydatidosis, the positive rate was 87. 0%(40/ 46) ,15. 6% (7/45)and 17. 8% (8/45) respectively. CAg were detected in 4 of 6 patients with CAb false negative reaction. These studies indicated that by combining tests for detection of CAb to- gether with CAg the positive rate may be improved up to 95. 7%(44/46). Pulmonary hydatidosis predominated in the patients with CAb negative reaction,it accounted for 83. 3%(5/6).The posi- tive rate of CAg was only 15. 5%,but the specificity was very high and no cross reaction and false positive reaction was found. It suggests that in case of detecting the CIC not only the positivity is very low but also the non-specific reaction is as high as CAb.更多还原