【摘要】 用荧光染料Fura-2测定了家兔肺泡巨噬细胞（AM）和人红细胞（RBC）胞浆游离钙离子浓度([Ca²⁺]₁),用furaptra测定了AM胞浆游离镁离子浓度([Mg²⁺]₁)。石英对AM和RBC的毒性导致[Ca²⁺]₁增加,作用时间越长,[Ca²⁺]₁增加越多。同样条件下,染石英尘后的AM[Mg²⁺]₁和脂质过氧化物（LPO）值也升高。以上结果提示石英可能破坏了细胞膜的结构和功能,以致细胞外Ca²⁺和Mg²⁺内流。卵磷脂、PVPNO和柠檬酸铝可拮抗石英引起的脂质过氧化作用和[Ca²+]₁及[Mg²+]₁的增加。卵磷脂有可能用于预防石英粉尘的毒作用。更多还原

【Abstract】 The intracellular free Ca²⁺ concentration ([Ca²⁺]i) of rabbit alveolar macrophages（AM）and human RBCwas determined with fluorence dye Fura -2.The intracellular free Mg²⁺ concentration of AM was determined withFuraptra.The toxic effect of quartz on AM and RBC leaded to the increase of [Ca²⁺]i.The longer the time of action,the greater the increase of [Ca²⁺]i.In the same case,[Mg²⁺]i and the LPO values also increased when AMwere exposed to quartz.It is demonstrated that quartz may cause an injury to the structure and function of cellmembrane.As a result,it causes the inner flow of extracellular Ca²⁺ and Mg²⁺.Lecithin,PVPNO and aluminum cit-rate can inhibit the lipid peroxidation and the increase of [Ca²⁺]i or [Mg²⁺]i which were induced by quartz.Lecithin may be useful to prevent the toxic effect of quartz.更多还原