【摘要】 本文采用简便的RP—HPLC 条件和萃取方法,9min 内同时测定微量血清（<100μl）中7种中枢神经抑制药。样品用三氯甲烷/乙酸乙酯(100∶2v/v萃取,N₂60℃吹干。色谱柱:Spherisorb-C₁₈（4.0mm×150mm）,流动相为MeOH/H₂O（45/55v/v）1.0ml/min 在220nm 检测。最小检出限≤35ng/ml,回收率在93.1%～98.3%,变异系数=1.6%。本法简便、快速、灵敏、准确。适于临床对配伍或交叉用药时的血浓度监测。更多还原

【Abstract】 A method for the detemination of seven central nervous inhibitory drugs,primidone,phenobarbital,fenarol.Phenytoin,amobarbital,nitrazepam and carbamazepine is reported.Serum sample was extracted with chloroform-ethly acetale（100:2v/v）.The organic phase was detemined by RP-HPLC with UV detector at 220nm and a spherisorb-C₁₈4×150mm（5μ）column.The mobile phase was CH₃OH-H₂O 45:55 V/V.The detection limit was<35ng/ml.Recoveries from serums weremore than 93%.This method was proved to be useful in clinic drug monitoring.更多还原