【摘要】 用改进的细胞遗传学方法制备染色体标本,鉴定了小鼠晚期桑椹胚(晚桑)、囊胚和扩展囊胚(扩囊)的性别。在实验中,利用小鼠早期胚胎染色体标本制备的理想实验参数,鉴定了242枚小鼠晚桑、囊胚和扩囊的性别,性别鉴定成功率分别为80.4％、99.0％和94.5％。以细胞遗传学方法鉴定小鼠早期胚胎性别的结果为标准,得出用间接免疫荧光法和PCR(聚合酶链反应)扩增SRY(y染色体的性别决定期)部分序列法分别鉴定100枚和26枚小鼠胚胎性别的准确率相应地为74.0％和92.3％。更多还原

【Abstract】 An improved cytogenetic. method for chromosome preparation was used for sexing mouse embryos in the stage of late morula ( LM ) . blastocyst ( B ) and expanded blastocyst ( EB ) . By using the optimal parameters for chromosome preparation of mouse preimplantation embryos in the experiment, 242 mouse embryos were sexed, the sexing rates in LM、 B and EB were 80.4%、 99.0%、 and 94.5% respectively. Compared to the figures in published papers available so far, these results were the best. The accuracy of mouse embryo sexing by indirect immunofluorescent assay and SRY sequence analysis with PCR was 74.07% ( 74/100 ) and 92.3% (24/26) respectively更多还原