【摘要】 以1.0％的Macerozyme R—10,1.5％的Cellulose Onozuka R—10,0.45mol/L的甘露醇和pH5.4的培养基作为分离番木瓜原生质体的最佳条件,得到了高活力和高产量的原生质体。PRV-Ys(Vb)株系和聚鸟氨酸(PLO,WW200000)分别以1μg/ml的最终浓度接种番木瓜原生质体(5×10~6原生质体/ml),成功地建立了PRV—番木瓜原生质体体系。对于PRV在番木瓜原生质体中复制的行为动态差异,本实验研究了PRV-Ys和PRV-Vb两株系,三个不同抗性梯度的番木瓜原生质以及不同培养温度之间相互作用,相互影响的关系,从而认为PRV不仅能在寄主品种Carica papaya var,F1和C.papaya var.Tw-5而且还能在非寄主C.stipulata的原生质体中进行增殖;环境温度不仅能影响PRV在番木瓜细胞中的增殖能力,而且还能影响植株细胞本身的生活能力和抗PRV侵染的能力;PRV-Ys和PRV-Vb两株系在C.papaya var.F1原生质体中的增殖潜伏期和增殖曲线没有明显差异。更多还原

【Abstract】 A concentration of 1×10~6 cells/ml of high viable protoplasts was obtained by using the following optimal concentrations for isolating papaya mesophyll cell protoplasts: 1.0% Macerozyme R-10, 1.5% cellulase Onozuka R-10 and 0.45 mol/L mannitol in the medium (pH5.5). The inoculation was successfully carried out by exposing 5×10~5 cells/ml of the protoplasts to the final mixture of 1 μg/ml PRV-Ys (or Vb) strains and 1μg/ml poly-L-orintine (MW200000). The preliminary results of the studies on behavior kinectics of PRV in protoplasts isolated from various resistant papaya varieties (species) and the effect of the culture temperature on the replication of PRV in protoplasts isolated from Carica papaya var. F1,showed that PRV-Ys strain could multiply not only in the protoplasts of the host plant,but also in those of non-host one. In the present study it was also found that the culture temperature affect not only the extent of multiplication of PRV in proto- plasts,but also the viability of protoplasts infected and the response of resistance to the infection of the intact plants by PRV. Otherwise, the replication inocubation periods of PRV-Ys and PRV-Vb strains in protoplasts isolated from C.papaya var.F1 bad not distinct difference.更多还原