【摘要】 本试验选用抗菌蛋白产生菌枯草芽孢杆菌(B.subtilis) TG26和晶体蛋白产生菌苏云金芽孢杆菌(B.thuringiesis subsp,pacifeus) AS1.904的营养缺陷型衍生株,在聚乙二醇的诱导下进行原生质体种间融合,获得了表现双亲遗传性状的种间融合菌株。融合率为7.52×10~6,融合子经传代10次,稳定率为19.5%。融合菌株的菌落和细胞形态与亲本株明显不同。通过SDS-聚丙烯酰胺凝胶电泳检测,融合菌株表达了亲本的抗菌蛋白和毒素蛋白。抑菌杀虫试验表明,融合重组菌株具有抑制多种植物病原菌和毒杀鳞翅目幼虫的能力。更多还原

【Abstract】 Protoplasts of two doubly auxotrophic strains, Bacillus subtilis TG26-10 producing antifungal proteins and Bacillus thuringeinsis AS 1.904-17 producing endoto-xin proteins, were fused by polyethylene glycol treatment. Interspecific fused reco-mbinants with parental genetical characteristics were obtained. The rate of interspecific fusion was 7.52 × 10-6. The obtained fusants were subcultured on MM medium plates for a.t lean 10 transfers and 19.5% of them were stable. There were apparent differences between parental strains and fusants in colony and cell morphology.The compositions of antifungal proteins and endotoxin proteins produced by parental strains and stable fusants were compared by SDS-polyacrylamide gel electr-ophoresis. The antifungal proteins from TG26-10 strain and the endotoxin proteins from AS1.904-17 strain were observed in the fusants patterns. The results of bioa-ssay indicated that the fusants were capable of killing both plant pathogens and larvae of Pyrausta nubilalis.更多还原