【摘要】 水稻IR36线粒体DNA经6种限制酶酶切,用脉冲电泳和长距离琼脂糖凝胶电泳分离酶切片段,获得高分辨率的清晰带型。每组酶切片段加和测得水稻IR36线粒体基因组大小分别为227kb(HindⅢ)、253kb(EcoRⅠ)、253kb(XhoⅠ)、294kb(BamHⅠ)、239kb(SalⅠ)和283kb(xbal)采用9个来自水稻和玉米线粒体基因组的基因探针与酶切条带杂交发现,水稻线粒体基因组含有包括编码基因在内的重复顺序。更多还原

【Abstract】 Combination of CHEF and electrophoresis on lengthened agarose gel was shown to have high resolution for HindⅢ, EcoRl, BamHl, Sail, xbal and Xhol restriction fragments of IR36 mt-DNA. The sum of th emolecular weight of f ra gments yields minimum molecular weight estimates of 227, 253, 253, 294, 239 and 283 kb for Hind Ⅲ, EcoRl, Xho\ BamHl, Sail and Xbol, respectively. 9 probes from mitochandrial genomes of rice and maize were used to hybridize restricted mtDNA of IR36. It was proved that rice mito genome contains repetitive sequences by their hybridization behavior.更多还原